Cellular Physiology and Dynamics

Research interest

1. Vascular Physiology.

  • Perivascular adipose tissue: vasoactive properties of adipokines.
  • Vasoactive effects of bioactive saffron compounds.
  • Macro- and microvascular function in the metabolic syndrome (SHROB rat).
  • Vascular effects of glitazones.
  • Expression of hypoxia-inducible factors in endothelial cells of human brain microvessels.

2. Development of novel non-invasive imaging techniques (from cells to whole organisms).

  • Luminescent indicators for calcium with red and infrared emission.
  • Fluorescent biosensor for phosphatidic acid.
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  • Título: Estudios in vitro e in vivo de la dinámica de calcio y ácido fosfatídico mediante indicadores recombinantes luminiscentes y fluorescentes
    Entidad financiadora: Ministerio de Economía y Competitividad
    I.P.: Juan Fco. Llopis Borrás
    Duración, desde: 01/01/2013   hasta: 31/12/2015
  • Título: Estudio de la dinámica y actividad enzimática de biomoléculas mediante sensores basados en proteínas fluorescentes
    Entidad financiadora: Consejería de Educación y Ciencia, JCCM
    I.P.: Juan Fco. Llopis Borrás
    Duración, desde: 01/04/2010   hasta: 31/12/2013
  • Título: Acciones de pioglitazona y rosiglitazona en aorta y microvasos en la rata espontaneamente hipertensa obesa (SHR-OB), un modelo animal de síndrome metabólico
    Entidad financiadora: Ministerio de Sanidad. FIS
    I.P.: Eduardo Nava Hernández
    Duración, desde: 01/01/2009   hasta: 30/06/2013
  • Título: Sensores para calcio, actividad proteasa de HCV y estudio de interacciones de PRP mediante transferencia de energía resonante
    Entidad financiadora: Ministerio de Ciencia e Innovación
    I.P.: Juan Fco. Llopis Borrás
    Duración, desde: 01/01/2009   hasta: 31/12/2012
  • Título: Efectos macro y microvasculares de las glitazonas en la rata espontáneamente hipertensa y en un modelo de síndrome metabólico
    Entidad financiadora: Consejería de Educación y Ciencia
    I.P.: Eduardo Nava Hernández
    Duración, desde: 01/04/2009   hasta: 31/12/2012
  • Título: Implicación de los astrocitos en neuroinflamación: funciones de NFAT
    Entidad financiadora: Consejería de Educación y Ciencia
    I.P.: Pedro A. Tranque Gómez
    Duración, desde: 01/04/2009   hasta: 31/03/2012
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  • Bakayan A1, Domingo B, Miyawaki A, Llopis J. Imaging Ca2+ activity in mammalian cells and zebrafish with a novel red-emitting aequorin variant. Pflugers Arch. 2015;467(9):2031-42. doi: 10.1007/s00424-014-1639-3. [Epub 2014]. (A).
  • Caminos E, Vaquero CF, Martínez-Galan JR. Relationship between rat retinal degeneration and potassium channel KCNQ5 expression. Exp Eye Res. 2015; 131:1-11. doi: 10.1016/j.exer.2014.12.009. [Epub 2014]. (A).
  • Llorens S, Arribas E, Arroyo MM, Artacho E, Carmona M, Domingo B, Fernández M, Insausti R, Jordán J, Legidos ME, Marcos P, Mohedano A, Muñoz M, Vaquero CF, Nájera A. Evaluation of the possible impact of clickers on the grade obtained by studens in the radiology subject, at the Faculty of Medicine in Albacete. En: INTED2014 Proceedings. 2014; 2707-2714. Ed. INTED. ISBN: 978-84-616-8412-0.. (CL).
  • Domingo B, Arribas E, Arroyo MM, Artacho E, Carmona M, Fernández M, Insausti R, Jordán J, Lejidos ME, Marcos P, Mohedano A, Muñoz M, Nájera A, Vaquero CF, Llorens S. The impact of concept-test activities with classroom response systems (clickers) in physiology in Medicine School of Albacete. En: INTED2014 Proceedings. 2014; 2761-2767. Ed. INTED. ISBN: 978-84-616-8412-0.. (CL).
  • Festuccia C, Mancini A, Gravina GL, Scarsella L, Llorens S, Alonso GL, Tatone C, Di Cesare E, Jannini EA, Lenzi A, D’Alessandro AM, Carmona M. Antitumor effects of saffron-derived carotenoids in prostate cancer cell models.Biomed Res Int. 2014;2014:135048. doi: 10.1155/2014/135048. (A).
  • Mancini A, Serrano-Díaz J, Nava E, D’Alessandro AM, Alonso GL, Carmona M, Llorens S. Crocetin, a Carotenoid Derived from Saffron ( Crocus sativus L.), Improves Acetylcholine-Induced Vascular Relaxation in Hypertension.J Vasc Res. 2014;51(5):393-404. doi: 10.1159/000368930. (A).
  • Caminos E, Vaquero CF, García-Olmo DC. ‘Green Mice’ display limitations in enhanced green fluorescent protein expression in retina and optic nerve cells. Histol Histopathol. 2014; 29(12):1601-12. (A).
  • Ferraz-Nogueira JP, Díez-Guerra FJ, Llopis J. Visualization of phosphatidic Acid fluctuations in the plasma membrane of living cells. PLoS One. 2014; 9[7]:e102526. (A).
  • Mendizábal Y, Llorens S, Nava E. Vasoactive effects of prostaglandins from the perivascular fat of mesenteric resistance arteries in WKY and SHROB rats. Life Sci. 2013;93(25-26):1023-32. doi: 10.1016/j.lfs.2013.10.021. (A).
  • Mendizábal Y, Llorens S, Nava E. Hypertension in metabolic syndrome: vascular pathophysiology. Int J Hypertens. 2013; 2013:230868. [doi: 10.1155/2013/230868]. (A).
  • Lisa S, Domingo B, Martinez J, Gilch S, Llopis JF, Schatzl HM, Gasset M. Failure of prion protein oxidative folding guides the formation of toxic transmembrane forms. J Biol Chem. 2012; 287(44):36693-36701. (A).
  • Prieto-Martín AI, Llorens S, Pardal-Fernández JM, Muñoz LJ, López DE, Escribano J, Nava E, de Cabo C. Opposite caudal versus rostral brain nitric oxide synthase response to generalized seizures in a novel rodent model of reflex epilepsy. Life Sci. 2012. 90[13-14]]: 531-537. (A).
  • Mendizábal Y, Llorens S, Nava E. Reactivity of the aorta and mesenteric resistance arteries from the obese spontaneously hypertensive rat: effects of glitazones. Am J Physiol Heart Circ Physiol. 2011. 301[4]: 1319-1330. (A).
  • Mendizábal Y, Llorens S, Nava E. Effects of pioglitazone and rosiglitazone on vascular function of mesenteric resistance arteries in rat genetic hypertension. Pharmacology. 2011. 88[1-2]: 72-81. (A).
  • Sabariegos R, Mas A, Clemente-Casares P. Population Diversity and its Relationship with Infectious and Tumor Diseases. Current Immunology Reviews. 2011. 7: 50-56. (A).
  • Bakayan A, Vaquero CF, Picazo F, Llopis J. Red fluorescent protein-aequorin fusions as improved bioluminescent ca reporters in single cells and mice. PLoS One. 2011. 6[5]: 19520. (A).
  • Vitali M, Picazo F, Prokazov Y, Duci A, Turbin E, Götze C, Llopis J, Hartig R, Visser AJ, Zuschratter W. Wide-Field Multi-Parameter FLIM: Long-Term Minimal Invasive Observation of Proteins in Living Cells. PloS One. 2011. 6[2]: 15820. [Epub 2010]. (A).
  • Picazo F, Domingo B, Pérez-Ortiz JM, Llopis J. Imaging local estrogen production in single living cells with recombinant fluorescent indicators. Biosens Bioelectron. 2011. 26[5]: 2147-2153. [Epub 2010]. (A).
  • Serrano-Pérez MC, Martín ED, Vaquero CF, Azcoitia I, Calvo S, Cano E, Tranque P. Response of transcription factor NFATc3 to excitotoxic and traumatic brain insults: Identification of a subpopulation of reactive astrocytes. Glia. 2010. 59[1]: 94-107. (A).
  • Domingo B, Gasset M, Durán-Prado M, Castaño JP, Serrano A, Fischer T, Llopis J. Discrimination between alternate membrane protein topologies in living cells using GFP/YFP tagging and pH exchange. Cell Mol Life Sci. 2010. 67[9]: 3345-3354. (A).
  • Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA , Llopis J. Fluorescence Resonance Energy Transfer-Based Assay for Characterization of Hepatitis C Virus NS3-4A Protease Activity in Live Cells. Antimicrobial Agents and Chemotherapy. 2009. 53[2]: 728-734. [Epub 2008]. (A).
  • Manjarres IM, Chamero P, Domingo B, Molina F, Llopis J, Alonso MT, Garcia-Sancho J. Red and green aequorins for simultaneous monitoring of Ca(2+) signals from two different organelles. Pflugers Arch. 2008. 455[5]: 961-970. [Epub 2007]. (A).
  • Pérez-Ortiz JM, Llorens S, Llopis J, Tranque PA, Nava E. Potential benefits of glitazones for cancer and vascular disease. Current Drug Therapy. 2008. 3: 111-125. (A).
  • Burgos M, Pastor MD, Gonzalez JC, Martinez-Galan JR, Vaquero CF, Fradejas N, Benavides A, Hernandez-Guijo JM, Tranque P, Calvo S. PKCepsilon upregulates voltage-dependent calcium channels in cultured astrocytes.Glia. 2007. 55[14]: 1437-1448. (A).
  • Llorens S, Fernandez AP, Nava E. Cardiovascular and renal alterations on the nitric oxide pathway in spontaneous hypertension and ageing. Clin Hemorheol Microcirc. 2007. 37[1-2]: 149-156. (A).
  • Llorens S, de Mera RM, Pascual A, Prieto-Martin A, Mendizabal Y, de Cabo C, Nava E, Jordan J. The senescence-accelerated mouse (SAM-P8) as a model for the study of vascular functional alterations during aging.Biogerontology. 2007. 8[6]: 663-672. (A).
  • Llorens S, Mendizabal Y, Nava E. Effects of pioglitazone and rosiglitazone on aortic vascular function in rat genetic hypertension. Eur J Pharmacol. 2007. 575[1-3]: 105-112. (A).
  • Martinez N, Garcia-Dominguez CA, Domingo B, Oliva JL, Zarich N, Sanchez A, Gutierrez-Eisman S, Llopis J, Rojas JM. Sprouty2 binds Grb2 at two different proline-rich regions, and the mechanism of ERK inhibition is independent of this interaction. Cell Signal. 2007. 19[11]: 2277-2285. (A).
  • Domingo B, Sabariegos R, Picazo F, Llopis J. Imaging FRET standards by steady-state fluorescence and lifetime methods. Microsc Res Tech. 2007. 70[12]: 1010-1021. (A).
  • Burgos M, Calvo S, Molina F, Vaquero CF, Samarel A, Llopis J, Tranque P. PKCepsilon induces astrocyte stellation by modulating multiple cytoskeletal proteins and interacting with Rho A signalling pathways: implications for neuroinflammation. Eur J Neurosci. 2007. 25[4]: 1069-1078. (A).
  • Perez-Ortiz JM, Tranque P, Burgos M, Vaquero CF, Llopis J. Glitazones induce astroglioma cell death by releasing reactive oxygen species from mitochondria: modulation of cytotoxicity by nitric oxide. Mol Pharmacol. 2007. 72[2]: 407-417. (A).
  • Marrachelli VG, Miranda FJ, Alabadi JA, Llorens S, Alborch E. Contribution of endothelin receptors and cyclooxygenase-derivatives to the altered response of the rabbit renal artery to endothelin-1 in diabetes. Eur J Pharmacol. 2006. 534[1-3]: 178-186. (A).
  • Gaietta GM, Giepmans BN, Deerinck TJ, Smith WB, Ngan L, Llopis J, Adams SR, Tsien RY, Ellisman MH. Golgi twins in late mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy. Proc Natl Acad Sci U S A. 2006. 103[47]: 17777-17782. (A).
  • Lazaro-Dieguez F, Jimenez N, Barth H, Koster AJ, Renau-Piqueras J, Llopis JL, Burger KN, Egea G.. Actin filaments are involved in the maintenance of Golgi cisternae morphology and intra-Golgi pH. Cell Motil Cytoskeleton. 2006. 63[12]: 778-791. (A).
  • Llorens S, Salazar FJ, Nava E. Assessment of the nitric oxide system in the heart, aorta and kidney of aged Wistar-Kyoto and spontaneously hypertensive rats. J Hypertens. 2005. 23[8]: 1507-1514. (A).
  • Vaquero CF, Domingo B, Picazo F, Pérez-Ortíz JM, Tranque P, Llopis J. Análisis de la dinámica celular con proteínas fluorescentes. Biojournal.net. 2005. 1. (A).
  • Perez-Ortiz JM, Tranque P, Vaquero CF, Domingo B, Molina F, Calvo S, Jordan J, Cena V, Llopis J. Glitazones differentially regulate primary astrocyte and glioma cell survival. Involvement of reactive oxygen species and peroxisome proliferator-activated receptor-gamma. J Biol Chem. 2004. 279[10]: 8976-8985. [Epub 2003]. (A).
  • Llorens S, Nava E. Endotelio, hipertensión arterial y aterosclerosis. Monocardio. 2003. : 161-166. (A).
  • Llorens S, Nava E. Cardiovascular diseases and the nitric oxide pathway. Curr Vasc Pharmacol. 2003. 1: 335-346. (A).
  • Nava, E. Endotelio vascular, óxido nítrico y presión arterial. Fisiología. Boletín de la SECF. 2002. 5 [1]. (A).
  • Nava E. Actualización y puesta al día en Fisiología: Endotelio vascular, óxido nítrico y presión arterial. Boletín informativo de la SECF. 2002. : 14-18. (A).
  • Llorens S, Jordan J, Nava E. The nitric oxide pathway in the cardiovascular system. J Physiol Biochem. 2002. 58[3]: 159-168. (A).
  • Moreno C, Lopez A, Llinas MT, Rodriguez F, Lopez-Farre A, Nava E, Salazar FJ. Changes in NOS activity and protein expression during acute and prolonged ANG II administration. Am J Physiol Regul Integr Comp Physiol. 2002. 282[1]: 31-37. (A).
  • Adams SR, Campbell RE, Gross LA, Martin BR, Walkup GK, Yao Y, Llopis J, Tsien RY. New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications. J Am Chem Soc. 2002. 124[21]: 6063-6076. (A).
  • Miyawaky A, Llopis JF, Mizuno H, Jalink K, Tsien R.. Cameleons as cytosolic and intra-organellar calcium probes.(2001). Págs: 3-16. ISBN: 0199638470. Ed. Oxford Univ. Press (Oxford, Reino Unido). (CL).
  • R Corti, I Sudano, C binggeli, E Nava, G Noll, TF Lüscher. Endothelial dysfunction and hypertension. (2000). Págs: 109-128. ISBN: . Ed. Wharwood Academic Publishers (Amsterdam, Holanda). (CL).
  • Matsuyama S, Llopis J, Deveraux QL, Tsien RY, Reed JC. Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis. Nat Cell Biol. 2000. 2[6]: 318-325. (A).
  • Balsinde J, Balboa MA, Li WH, Llopis J, Dennis EA. Cellular regulation of cytosolic group IV phospholipase A2 by phosphatidylinositol bisphosphate levels. J Immunol. 2000. 164[10]: 5398-5402. (A).
  • Nava E, Moreno C, Salazar FJ. Increased constitutive nitric oxide synthase activity in the aged heart. Effects of hypertension. (2000). Págs: 65-65. ISBN: 1 85578 142 5. Ed. Portland Press (Londres, Reino Unido). (CL).
  • Nava E, Salazar FJ, Bentura ML, Uttenthal O, Fernández AP, Serrano J, Rodrigo J.. Intrarenal distribution of constitutive nitric oxide synthase and nitrotyrosine in spontaneously hypertnesive rats. (2000). Págs: 68-68. ISBN: 1 85578 142 5. Ed. Portland Press (Londres, Reino Unido). (CL).
  • Nava E, Rodriguez F, Moreno C, Llinas MT, Salazar FJ. Release of nitric oxide after acute hypertension. J Cardiovasc Pharmacol. 2000. 36[4]: 444-451. (A).

Juan F. Llopis Borrás



Permanent staff

Phone: (+34) 926 295 300

Contact information

Facultad de Medicina de Albacete

Universidad de Castilla-La Mancha

C/ Almansa, 14.

02008 Albacete (España)

telefono (-34) 967 599 200, Ext. 2935

fax (+34) 967 599 327

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